Anyone have a good protocol for extracting 4,4'-(1H-1,2,4-Triazol-1-ylmethylene)dibenzonitrile from Letrozole drug?

Can anyone give the detailed procedure for extraction.

30 December 2020 513 1 View

I am Looking For Spam Dataset ?

I am Looking For Spam Dataset? Can I find a comprehensive Dataset with.csv or .arff extensions which compatible with Weka?

03 December 2020 5,254 3 View

No title

08 November 2020 7,455 5 View

The second wave of the virus is Covid 20 !?

The second wave of the virus is Covid Does the virus develop and become more lethal and infect the lower part, i.e. the stomach and intestine, with severe diarrhea, intestinal bleeding and ulcers...

02 October 2020 4,767 1 View

How to discuss an insignificant p-value?

I have a small sample size (29 patients) and divided them into two groups each of which has a different sample size. I have an ordinal scale (dependant variable ) which is a scoring system to...

13 September 2020 7,298 31 View

What made English to become the language no. 1 in the world?

Why English in particular? Why not French, German, Arabic, Spanish, Chinese, or any other language?

27 July 2020 7,754 90 View

What is the most reliable method for performing ELISA test on mouse brain?

Dear fellow researchers, may I ask you for your advice regarding performing ELISA test on mouse brain? which method gives a better yield snap freezing the tissue in liquid nitrogen, storing it...

26 July 2020 1,376 3 View

How to calculate Stress intensity factor in ABAQUS XFEM with allowing for crack growth?

Dear All, I want to model crack growth in a plate using ABAQUS XFEM method. Here is my question about it: How to calculate Stress intensity factor in XFEM when crack growth in ON? At this time,...

17 July 2020 1,341 1 View

Scientific relationship between stress intensity factor (SIF) and crack tip opening displacement (CTOD)?

Dear connections , What is the scientific relationship between stress intensity factor (SIF) and crack tip opening displacement (CTOD) with specimen double edge cracked. So really I need your...

13 June 2020 9,426 1 View

How to keep in memory the global ecological pause during the COVID-19?

Dear Researchers, I would like to raise a debate with you, regarding the impact of COVID-19 in the environment and climate change. We are a group of researchers that have launched a map, which...

08 June 2020 9,286 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Is it possible to perform cardiac puncture followed by PBS perfusion with a pump?

Hello all, I will be perfusing mice with PBS for brain harvest and a colleague would like to collect the blood via cardiac puncture (these are 18m old mice so would be nice to maximize use). Has...

15 February 2021 5,312 2 View

How to properly reach normality in residuals with lmer?

Hello, I have a large non-balanced panel dataset regarding a company dealing with private lessons for students. My goal is to fit a lmer model which predicts the number of monthly hours taken by a...

08 February 2021 2,539 7 View

Do you think that covid 19 virus separates through the air and make infections?

Do you think that covid 19 virus separates through the air and make infections?

14 September 2020 7,956 7 View

Any suggestions for a simple method to reduce the viscosity of bacterial growth media?

Hi, I am working with a bacteria from the genus Paenibacillus. I am having trouble separating it from the media (PDB), and difficulties in extraction of secondary metabolites from the media,...

04 August 2020 9,974 3 View

Can anyone suggest procedure to analyse solid state CD and UV-Vis spectra for chiral polymers?

To check CD and UV-Vis spectra for porous polymers.

28 May 2020 7,359 8 View

How can I improve my protein purification with Qiagen protocol-Ni-NTA column?

Hi, I purified a protein (about 28KD) via the following steps: 1- Firstly, I used buffer A native(Qiagen)-NaH2PO4,NACL,Immidazol,ph8 2-then after centrifuging and separating my pellet , I added...

13 May 2020 1,673 6 View

How to separate phospholipids from betaine lipids using silica chromatography?

I tried separating betaine lipid from phospholipid and galactolipid from my 2D TLC plate but to no avail. Betaine lipid was found to overlap with some galactolipids under the condition of:- 1D:...

25 March 2020 641 3 View

Do you think blood or bone marrow should be diluted before separating Buffy coat by LymphoSep?

First, do you think it is necessary to dilute blood or bone marrow samples before adding it on lymphoSep to separate the Buffy coat? OR simply add it on lymphoSep and then centrifuged. Second,...

04 March 2020 3,041 1 View

Sep-pak C18 cartridge alternatives?

I am currently conducting a study about the formation of free EPA in raw oysters treated with acidic sauces. For separating the polar and nonpolar lipids, I will be using Sep-pak C18 catridges....

08 February 2020 8,278 3 View