I WANT TO DO ANTIBIOTIC ASSAY. For sample (Shrimp Ethyl acetate extract) EVAPORATION I don't have Nitrogen evaporator, any alternate is their?

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Rob Darrington

Dear Annadurai,

Evaporating the solvent(s) under vaccum means that they are cold - the vacuum depresses the boiling point of the solvents, and it is a relatively quick process. Low temperature is better becuase it helps protect the analyte(s) of interest.

The attached paper may help

Kind regards

Rob

Jerry Decker

I agree with vacuum, because a high temperature would damage the biochemicals.

Eswar Annadurai

Dr.Jerry Decker

How much temperature we can maintain to evaporate sample?