I want to do 2D preparative thin layer chromatography and stain with iod for detection. How likely is the probability that my lipids will oxidize?

More Ann-Kristin Diederich's questions See All

Can I use two different ranges of 5-point Likert scale (0-4 and 1-5; adapted from two different authors) in my questionnaire?

Good day everyone! I have searched and read a lot of topics about this one and yet there are still varying answers left and right. I need a specific one so I hope someone can help me with this. I...

02 July 2024 1,772 2 View

How do I correlate two sets of 5 point Likert scale questions?

Hello everyone. I have 2 sets of questionnaires with the following scale choice: (1) Poor - Excellent (2) Seldom - Always Both are 5-point Likert scale. The first scale has 10 items, asking for...

22 May 2024 9,845 5 View

What is the research gap about the title topic?

title topic " Role of Facility Layout in enhancing workplace safety and ergonomics in Batangas State University"

06 May 2024 8,388 1 View

Please help. What is the name of the lock acquired before entering the soft interrupt in the Linux source code?

My experimental environment is Linux6.1, and the real-time patch preempt_rt has been applied to improve the real-time performance of the network. I checked the relevant papers and saw a lock...

18 April 2024 8,721 3 View

What is the relationship between anticoagulants used in blood collection tubes and lactate dehydrogenase (LDH) activity in plasma or serum samples?

This question is derived from an article entitled "Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in serum and heparin plasma samples" by Neuwinger...

08 April 2024 8,391 1 View

Does Sysmex DI-60 facilitate remote access for users in different geographical locations? Does it have features for troubleshooting?

I am interested in the functionality of the Sysmex DI-60 system in terms of its remote access capabilities and troubleshooting features. Particularly, I am curious to know whether this automated...

01 April 2024 5,516 1 View

Different species, same matrix, can I make different way to make STD and QC samples during method development and validation?

There days I was facing one challenge when doing MD and MV. I need to use rat and pig' plasma as matrix. I used to spike the solvent with different concentrations into the plasma to make STD and...

01 April 2024 3,766 0 View

What are students perspectives on school fights?

What do students think are the reasons for school fights

26 February 2024 4,633 5 View

Can the XRD intensity peak of magnesium doped ZnO be more than the undoped ZnO?

Can the reasons for such an incident be detailed?

13 February 2024 2,795 4 View

What are the preparations needed to be followed by the patient during the collection of their urine & how can microbiologist ensure that the patient?

The question is based on the article, “Tenets of Specimen Management in Diagnostic Microbiology” by Rajeshwar and Pathak. It aims to know how the microbiologist can help the patients do their part...

05 February 2024 9,935 3 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Roberto Angelini

Maybe you can find your answer here

Lars I Hellgren

Since the iodine binds to the double bonds, I have learned that it is a destructive methods that oxidizes the double bond. If you want to do fatty acid analyses after your separation, I recommend staining with 0.05% 2,7 dichlorofluorescein in ethanol. Lipid spots will fluoresce under UV, without affecting double bonds.