Hi
i formulate polymeric nano crystal using F127 and F68 by different ratio . these form gel at 38 c i want to decrease gelling temperature to 34 c. i need to add material to decrease gelling temperature.
Try a milk chocolate.
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Try to add salting-out salt such as (ammonium or potassium) (fluoride or phosphate).
Please note that comments of Alan F Rawle are roads for learning.
Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.
03 March 2021 7,174 5 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...
23 February 2021 3,175 3 View
I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use
21 February 2021 3,944 3 View
I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...
17 February 2021 7,402 4 View
the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please
16 February 2021 8,801 3 View
There are contradictory research papers about the correlation between meteorological parameters and incidence of COVID-19 in some countries worldwide. Is there correlation between the...
15 February 2021 5,806 2 View
Are there alternatives to the Devarda alloy when estimating nitrogen? devarda alloy is reducing the nitrate to ammonium
15 February 2021 8,838 3 View
I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...
14 February 2021 3,142 7 View
Hi, A Fabry Perot Cavity allows to increase the directivity of a low gain antenna by putting in the top of cavity a superstrate with high permittivity such as a ceramic (er=10.2). However,...
09 February 2021 6,799 8 View
I anwar by adsorption or reaction ..but it is either more method...
02 March 2021 4,811 2 View
I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...
02 March 2021 7,626 1 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...
26 February 2021 4,026 5 View
In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...
26 February 2021 2,435 4 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View