I want to analyse pulse field gel electrophoresis data and generate the dendrogram. Can anyone suggest a website OR free software ?

05 January 2013 1 7K Report

I have different strains of Pseudomonas Aeruginosa and I want to see the relatively between each strain.

- check virtual band pattern: http://insilico.ehu.es/ for comparison

- copy banding pattern in Excel

- calculate distance matrix: Freetree (http://web.natur.cuni.cz/flegr/pdf/FL14-freetree.pdf; http://ijs.sgmjournals.org/content/51/3/731.long; http://ijs.sgmjournals.org/content/51/3/731/suppl/DC1)

- paste in TreeView (http://taxonomy.zoology.gla.ac.uk/rod/treeview.html)

Good luck

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