Extraction of RNA from FFPE tissue blocks?

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Using a large amount of data, such as 30 years of daily data, in a machine learning model like LSTM can reduce the model predictivity?

I have used 30 years of daily data, but the model is not able to optimize, getting just 0.57 in NSE and R2. when I reduced the data to 20 years in daily time frame I got 0.73 in NSE, R2 for both...

22 July 2024 669 0 View

Could anyone recommend software for drawing the physical structure of nanoparticles?

I am looking for professional software to draw the physical structure of nanoparticles or vesicles, not the chemical structures for which we use ChemDraw, but the actual physical structures,...

23 June 2024 1,292 3 View

How could I analyze the results of caspase-glo 3/7 assay?

I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...

02 June 2024 7,833 2 View

How can I identify an unknown gene of known activity ?

Hi, I've observed a new biological activity in certain cells, likely induced by an enzyme. How can I determine the gene or genes responsible for expressing this unknown enzyme? Thanks in advance

31 May 2024 9,111 3 View

Hello everyone, I am looking for collaboration to do clinical research?

I have many skills like data analysis with R programming or Spss , scientific writing and paper publishing

23 May 2024 1,683 3 View

What is the best software for figures merge?

High resolution and preferably free with links. Thank inadvance

14 May 2024 291 0 View

Does GFP interferes with absorbance in MTT assay or luminescence in Caspase 3/7 Glo assay?

I have cells that produce GFP as a control, and I intend to perform the MTT and Caspase 3/7 Glo assays. The emission wavelength of GFP is 508 nm, and its absorption is 475 nm. In addition, I will...

16 April 2024 3,537 2 View

I would like to know why I obtained such false positive curves, which appears on both linear ang log scales healthy curves?

The positive curves for E coli different strains from mucual stool sample.

29 March 2024 5,907 0 View

Methylation of spata 6 gene in rat males?

methylation of spata 6 gene and its role on male infertility of rat

08 March 2024 9,667 0 View

How to prepare concentration MPs for field scale ( phytoremediation of floating plants ( water lily, water mimosa, water hyacinth )?

how to prepare concentration MPs for field scale ( phytoremediation of floating plants ( water lily, water mimosa, water hyacinth )?

04 March 2024 1,822 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Alaa Siddig

Thank you so much Busra.

Maroua Manai

I used the Qiagen one and it worked very well with me.

The quality will depend on the tumor fixation steps done in the pathology core/department.

Good luck!

Marwa

Thank you Marwa for your comment, if it's possible could you please share with me the protocol you have followed.