I transformed a plasmid into an Agrobacterium competent cells but i couldn't get any colonies in my plates. What's the reason?

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Reza Hadavi

you should prepare electro- competent cells and then electroporated them .

remember final antibiotic concentration should be 50 ug/mL (kanamycin)in medium .

https://bmb.natsci.msu.edu/_bmb/assets/File/Sharkey_lab/Agrobacterium%20Transformation%20and%20Competent%20Cell%20Preparation.pdf

Hêro Farhad Salah

Check the activity of the competent cells.