I am wondering if I should use CHX to inhibit translation and if message builds up that would answer the question that it is not degraded. However if anyone has expertise, i'd much appreciate any help.
My question is how ould you know your mRNA going down? Did you check the mRNA expression for a specific gene? If answer is yes then I should say sometimes it happens due to switch on off mechanism of gene.Once the mRNA is synthesized the expression of gene is off and the mRNA is expressed as protein. It is common in case of fungi. I got similar result.