Mustafa Alam

4 Questions 9 Answers 0 Followers

Questions related from Mustafa Alam

I observe mRNA going down upon exposure to a stimulant, however the protein is going up. I'm wondering if message has been degraded or translated.

I am wondering if I should use CHX to inhibit translation and if message builds up that would answer the question that it is not degraded. However if anyone has expertise, i'd much appreciate any...

18 December 2012 108 3 View

What is the difference between EIF2C AND EIF2C1?

I am wondering about the nomenclature of eukaryotic translation initiation factors. I do understand that these are different isoforms, but are they equally expressed? Do they have exactly same...

25 September 2012 5,154 0 View

L929 (derived m-csf) vs recombinant MCSF

I have observed numerous times that L929 cell media containing m-csf makes bone-marrow derived macrophages proliferate and mature much more nicely than recombinant mcsf available from the likes of...

29 August 2012 2,609 31 View

Chromatin Immunoprecipitation (ChIP Assay) - Protocol

Hi I'll be starting CHIP pretty soon, its going to be my first time and we don't have much support available in the lab. I would much appreciate if I could be given any advice, good protocol, tips?

13 April 2012 3,453 11 View