biology
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...
19 July 2024 4,868 1 View
Hello, I am trying to clone 1334 bp insert into 6.1 kb vector using HiFi DNA assmebly/Gibson assembly kit. I am taking 100ng of vector and 1:5 molar ratio of vector: insert and 4 hrs of...
27 June 2024 9,652 2 View
I am looking for a way to calculate the number of water molecules that are in a hydrogel sample.
16 May 2024 7,064 3 View
I performed two RAD-seq assemblies using ipyrad. I filtered the outputted .vcf file of each of the two assemblies using vcftools to limit the maximum quantity of missing data per locus to 60%. To...
12 March 2024 2,136 0 View
Articles about this topic, statistical methods and analisys for this. What socioeconomic factors are related with higher acces education?. Thanks.
06 December 2023 7,177 6 View
I am having a doubt regarding CMV promoter and SV40 promoter in mammalian expression vector 1. Why CMV and SV40 promoters are used in mammalian expression vectors why not others? 2. There is a CMV...
05 December 2023 1,830 3 View
Me gustaría subir a ResearchGate mi nueva publicación en el formato de pdf Psicoteología: la neurociencia de la fe, para la difusión y divulgación del contenido. Gracias.
05 November 2023 7,352 1 View
El enfoque por competencias en educación, durante los últimos años ha alcanzado gran relieve en los diversos proyectos educativos, sobre todo, porque hay muchos modelos educativos que se han...
11 June 2023 3,550 2 View
Hello, I am trying to achieve the CRISPR KNOCK IN of HDR 1.7 kb in HEK293T will one sgRNA will be efficient to get a knock in or else should I use two sgRNAs?
19 May 2023 9,990 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View
