OD600 of Pichia stipitis?

25 September 2013 0 7K Report

Can someone please tell me what the OD at 600 nm = 1 equals (how many cells/ml) for Pichia stipitis?

Badges
Science topic

More Nirosha Pillay's questions See All

The isolation of genomic DNA from fermented milk samples has resulted in smears, what could be the cause?

Hi all, there have been some challenges with regards to genomic DNA extraction directly from fermented milk samples. Any input with knowledge on troubleshooting would be appreciated. I have been...

07 September 2023 394 2 View

What are the best areas to conducting a research for the postgraduate students who are following degree in MBA E-Governance ?

Generally students are just picking some research areas and conducting a research for their final Thesis. What are the best topics that student can choose in their MBA E-Governance post graduate...

22 October 2021 7,876 10 View

Factor Analysis with items measured on different scales?

In my newly developed tool with items measured on 10 point and 4 point likert scales. How can I do FA. Am I supposed to standardize the scores..? otherwise can I do FA without standardize..?...

20 February 2021 2,362 3 View

Removing an item from a scale to increase the cronbach's alpha?

Hello fellow academics. So i have been struggling with a very low cronbach's alpha on a sub scale i.e alpha of .383 to be precise. After following the advice of some academics on research gate i...

05 July 2020 6,696 1 View

I need some assistance in sourcing a supplier for nylon or Dacron tipped non-wooden swabs for Covid-19 testing?

Hi nylon or Dacron tipped non-wooden swab.

13 May 2020 419 0 View

I need to stain protoplast with Rhodamine B. Pls advice me to prepare Rhodamine for protoplast staining and observation?

How to prepare Rhodamine B for staining protoplast? How to stain? What is the excitation level i should use to observe stained protoplast?

27 November 2017 2,885 3 View

Further management: mCRC with DPG deficiency?

Hi, does anyone have an information regarding further treatment options for a 61 year old male with mCRC with secondaries to the left lung and mediastinal nodes who has DPG deficiency confirmed...

03 September 2017 1,608 1 View

Please help. I have been trying to grow Chlorella vulgaris in 5g/L glucose in BG-11 medium. It grows for 3 days and then dies. Any reasons?

I have tried this about 4 times and it shows the same pattern. In the presence of sucrose, fructose etc., growth continues after 3 days. But in glucose, it just won't grow. All articles indicates...

11 August 2017 2,275 0 View

Have any of you used, or are aware of, pragmatism being used as a theoretical framework for conducting research?

We are aiming to use pragmatism for conducting research in the field of Occuaptional health and safety. I understand this is the preferred theoretical framework for conducting mixed-method...

17 July 2017 6,391 3 View

Anyone aware of research on the impact of mindfulness training on vicarious trauma in mental health care workers?

Seems to be a dearth of academic research on mindfulness interventions in the South African context

23 April 2014 8,133 10 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View