i need help with my protein expression (AGTR1), i have successfully cloned this protein into a pGEX vector. overexpressed the vector (GST) successfully at 37 degress but cant seem to achieve the overexpression the clone (AGTR1-GST).

I have tested my antibiotics and IPTG and confirmed them to be effective. i think the challenge may be related to a suitable temperature for growth.

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