Wisdom John Okoye

3 Questions 3 Answers 0 Followers

Questions related from Wisdom John Okoye

Has anyone used a LightCycler Nano PCR machine to do a High Resolution Melting Thermal Shift Assay?

I am trying to do a high resolution melting thermal shift assay by applying a thermal shift assay control kit, to a LightCycler Nano Instrument from Roche. I cant seem to get anything off the...

09 September 2018 6,668 2 View

How do I deal with recombinant protein seperation after MBP Factor Xa cleavage digestion of recombinant fusion protein?

After purifying my recombination fusion protein sample using a Bio-rad Q-column chromatography, the resultant samples, were verified via SDS-PAGE analysis and stored in glycerol 20% concentration...

07 July 2018 5,534 4 View

I need help with my protein expression, i have successfully cloned this protein expressed the vector (GST) successfully at 37degrees any help please?

i need help with my protein expression (AGTR1), i have successfully cloned this protein into a pGEX vector. overexpressed the vector (GST) successfully at 37 degress but cant seem...

10 October 2016 4,287 4 View