I need help How to caluclate the mean SD of the experiments performed in triplicate?

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Which transport medium is best for E.coli?

I'm working on Microbiology EQA program. I have confirmed E.coli strains in my lab. Now I want to transport them to other labs at a distance of 10-12 hrs. we don't want to use ice-packs during...

23 July 2024 1,855 0 View

How to calculate the mass of an elliptical galaxy?

Hello, I would like to know how to calculate the mass of an elliptical galaxy. If someone could explain the commonly used methods and formulas, it would be greatly appreciated. Additionally, could...

02 June 2024 3,239 10 View

In regards to adding diodes at the input (or output) of choppers in series and parallel modular topologies?

Hello I would like to know : In the context of photovoltaic systems, if it is essential to integrate diodes into modular series and parallel chopper topologies? What is the significance of this...

11 May 2024 4,040 4 View

Hi I am a new in VASP. I have done some electronic calculations like DOS and band structure. Now i want to do calculate vibrational DOS of LI7P3S11?

I want to know what changes i need to make for relaxing the structure for vibrational density of states and what is best way to proceed

05 March 2024 854 1 View

What other variables should I add with this topic for PhD? My topic is “effect of organisation cynicism on organisation citizenship behavior?

I worked on this topic in my master thesis now I want to work on this topic with addition of some more variables for phd,what variables would you guys suggest? thank you.

11 February 2024 2,762 1 View

Can we use DH5-alpha for conjugation assays (mcr gene transmission)?

I'm working on HGT of colistin resistance in E.coli. Is In most of the papers, E.coli J53 is used, I don't have that strain , can I use DH5-alpha for this purpose. Normally E.coli J53 has sodium...

31 January 2024 916 0 View

How does emphasis on years of teaching experience vary between faculty recruitment practices in Malaysian and Pakistani higher education institution?

How does the emphasis on years of teaching experience vary between faculty recruitment practices in Malaysian and Pakistani higher education institutions?

28 January 2024 2,423 4 View

2. How do you think academic culture can influence teaching practices?

1. How do you think academic culture can influence teaching practices?

24 January 2024 5,739 10 View

How to troubleshoot Easyfig?

I'm working on comparative genomics. I've to compare plasmids in terms of genes. In most of the papers, I've read about Easyfig. Initially, I faced trouble with blast downloading, later I...

20 January 2024 1,846 1 View

Need E.coli J53 for conjugation assays, where to get it from Pakistan?

I'm performing conjugation assays in E.coli. I need E.coli J53 as recipient strain. This strain is not available in my university. If anyone have this strain in their lab kindly tell me. thankyou

13 January 2024 7,552 0 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

How to separate the stereoisomers compond in reaction mixture?

how to separate the organic reaction stereo isomers compounds are separate from the reaction mixture any simple methods are available

23 July 2024 8,025 4 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View