I'm performing conjugation assays in E.coli. I need E.coli J53 as recipient strain. This strain is not available in my university. If anyone have this strain in their lab kindly tell me. thankyou
Hello, I hope everyone reading this is doing well during these tough times. I am doing my masters in business management and considering dissertation topics. I'm interested in taking an...
15 February 2021 594 26 View
I have images that I need to explain the color of the picture
02 December 2020 373 2 View
Does anyone have Matlab code to filter images? I am working to built modeling to image process that i need to filter image which show me clear to picture ,I can know the parameters of defect
02 December 2020 6,434 4 View
I have performed this reaction many times by using acetic acid as a solvent bt no cyclization occurs to form imide.
05 March 2020 6,051 3 View
acid can be converted into anhydride by sublimatiom?
05 March 2020 8,313 2 View
Dear colleagues, I have "leaf Area" measurements using the "LI-3100 Area meter" of cotton plants from different stages on June, July, August and September. The planting distance is 15 cm between...
12 February 2020 1,583 3 View
Hi I prepared a nanocomposite cathode LSCF-GDC and extracted its conductivity/resistivity data from Hall Effect Measurement System at room temperature. I am looking for a published paper, thesis...
18 December 2017 5,266 3 View
I am looking for a Post-Doctor position in Europe, USA or Canada for two years or more. Please if you have any suggestions or you have a position to offer you may contact me by e-mail :...
01 October 2017 5,145 1 View
I am trying to assess BTB integrity. Most articles show experiments with Biotin Tracer. But incase of Blood brain barrier Evans blue or albumin is used often. Why in case of BTB biotin tracer is...
27 September 2016 1,337 3 View
Basis/Principles of the signal secuences predictions
18 April 2016 9,042 1 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...
25 February 2021 9,659 2 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View