I'm performing conjugation assays in E.coli. I need E.coli J53 as recipient strain. This strain is not available in my university. If anyone have this strain in their lab kindly tell me. thankyou
I'm working on Microbiology EQA program. I have confirmed E.coli strains in my lab. Now I want to transport them to other labs at a distance of 10-12 hrs. we don't want to use ice-packs during...
23 July 2024 1,855 0 View
Hello, I would like to know how to calculate the mass of an elliptical galaxy. If someone could explain the commonly used methods and formulas, it would be greatly appreciated. Additionally, could...
02 June 2024 3,239 10 View
Hello I would like to know : In the context of photovoltaic systems, if it is essential to integrate diodes into modular series and parallel chopper topologies? What is the significance of this...
11 May 2024 4,040 4 View
I want to know what changes i need to make for relaxing the structure for vibrational density of states and what is best way to proceed
05 March 2024 854 1 View
I worked on this topic in my master thesis now I want to work on this topic with addition of some more variables for phd,what variables would you guys suggest? thank you.
11 February 2024 2,762 1 View
I'm working on HGT of colistin resistance in E.coli. Is In most of the papers, E.coli J53 is used, I don't have that strain , can I use DH5-alpha for this purpose. Normally E.coli J53 has sodium...
31 January 2024 916 0 View
How does the emphasis on years of teaching experience vary between faculty recruitment practices in Malaysian and Pakistani higher education institutions?
28 January 2024 2,423 4 View
1. How do you think academic culture can influence teaching practices?
24 January 2024 5,739 10 View
I'm working on comparative genomics. I've to compare plasmids in terms of genes. In most of the papers, I've read about Easyfig. Initially, I faced trouble with blast downloading, later I...
20 January 2024 1,846 1 View
I'm working on HGT of colistin resistance through plasmids. I've done plasmid typing and mapping. I've samples from different poultry sources. To perfrom comparative genomics, I started learning...
13 January 2024 7,139 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?
23 July 2024 8,008 1 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View