Alexander Hoffmann

2 Questions 2 Answers 0 Followers

Questions related from Alexander Hoffmann

Can Chelex 100 remove transferrin bound iron and ferritin bound iron from plasma?

I would like to have iron free FCS for cell culture. Is Chelex capable to remove not just free iron but as well transferrin and ferritin bound iron from FCS? If not. Does anyone has...

11 October 2021 4,138 0 View

How do i get my RAW cells to engulfs erythrocytes?

I use RAW cells in DMEM + 10%FCS and "feed" them with heat treated (20 min 48C°) clatrin stained erythrocytes. I stimulate the RAW cells with 50ng/ml LPS and incubate the RAW cells and erys for...

09 March 2017 336 1 View