Hello Elysha Nur Ismail

Insulin stimulates the growth and proliferation of a variety of cells in culture. In cell culture, it interacts synergistically with other hormones and growth factors such as PDGF, FGF, EGF, tumor-promoting phorbol esters, and thrombin, to stimulate progression through the cell cycle. In some cells, the growth-promoting effects of insulin appear to be mediated primarily by its low affinity interaction with receptors for insulin-like growth factor I (IGF-I), while in others, insulin appears to stimulate growth by binding to high affinity insulin receptors.

The insulin and IGF-I receptor proteins, like the receptor proteins for other growth promoting hormones such as EGF and PDGF, are closely associated with tyrosine-specific protein kinase activities. The mechanism by which the binding of insulin to its receptor and activation of the receptor-associated tyrosine protein kinase activity control intracellular protein phosphorylation and dephosphorylation reactions, help in the passage of cells through the phases of the cell cycle.

So, insulin forms an important constituent of the growth media that can help in the optimal growth of FHs74 cells. If you do not add insulin in the media, you may not observe optimal growth. If you are planning to use these cells for cell-based assays, they must be in good condition otherwise you may end up with unreliable results.

Best.