I haver to conjugate aptamer with nanoparticle using EDC/NHS protocol. Can anyone suggest me whether the NH2 group needs to be added to 5' or 3' end ?

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How to get logarithmic colorbar in MATLAB R2012a for 3D surface plot?

I am trying to plot a 3d surface which has two axes in log scale. After the getting plot and x & z axes converted into log scale, colorbar is not changing linear to log scale. Please help me to...

04 February 2021 7,290 2 View

Plasmid DNA sequencing showed no insert gene inside vector but PCR using gene specific primers showed good band. Is it possible?

I am trying to clone HA gene of influenza virus into the phw2000 vector. Top-10 cells were used for transformation. A few colonies tested positive using gene specific primers were processed for...

08 October 2020 2,825 7 View

No protein expression in Pichia, any ideas?

I introduced a heterologous gene using pPIC 9 plasmid into KM71 strain and confirmed the presence of the target gene by PCR. The colonies (6) I am checking growing well on Minimal growth media...

25 May 2020 1,611 9 View

What additional info should i include when i wanna use surveys ?

I am going to use human subject for my research proposal;survey . should i include any other information

07 May 2020 9,210 7 View

Which computational method- DFT, Molecular mechanics or Molecular dynamics provides more accurate structure?

I am working on cyclodextrin inclusion complexes and find out their geometries,particularly the orientation of the guest in the cavity. Which computational method out of DFT, MM and MD would...

06 September 2019 1,205 6 View

How the Cl history for pitching airfoil(i.e at changing AoA) can be calculated in Ansys (Fluent)?

Hello everyone... I need your views/ideas. I am having serious problem and got stuck and dont know how to encounter that issue. The master's thesis submission date is around the corner. My...

25 October 2017 5,871 6 View

How to calculate the group velocity using Phonopy?

Dear all, I used the tags in "mesh.conf" file DIM = 2 2 2 Band = ............... GROUP_VELOCITY = .TRUE. and then used the command "phonopy -p --gv mesh.conf" it gave me the graph of band...

03 March 2017 8,855 2 View

I have an unbalanced dataset, which classification Measure is better, F-Measure or Accuracy?

Which Measure gives accurate results

16 July 2014 2,708 2 View

What is the effect of inlet air temperature on the morphology of powder particles using protein as a carrier?

I am using protein as carrier in spray drying of juice powder.

04 June 2014 5,073 3 View

Is there any correlation between I.Q and Achievement?

If the achievement is affected by the I.Q then why do some Developmental Disorders (DD) show low achievement in spite of high I.Q?

01 January 1970 2,717 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View