I haver to conjugate aptamer with nanoparticle using EDC/NHS protocol. Can anyone suggest me whether the NH2 group needs to be added to 5' or 3' end ?

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Omar Gonzalez-Ortega

Well, I do not know the answer to your question, from the chemical point of view, the amino group must be available to react (no steric hindrance). Good luck using EDC/NHS, it does not work.

Regards

Aneesa Muzaffar

Thank you for your suggestion

Does the protocol for conjugation using EDC/NHS not work? Omar Gonzalez-Ortega

It is very inefficient as hydrolysis is the enemy.

Thank you