Antibody : loss of binding at high concentration (beyond saturation) Why?

Hi everyone, It is commonly observed that some antibodies presents a binding saturation curve that goes down at high concentration (at concentration beyond saturation). Does anybody have a good...

20 August 2020 778 5 View

Can authors names in a publish paper influence the visibility of that particular article?

Can the position, grade, number of publication of scientists that authored a paper influence the visibility (acceptance, read, recommendation, citation,....) of an article during the submitting...

14 July 2020 3,011 8 View

What is the more adapted protocol for rat testes using NBF solution?

Hi all, I have found loads of paper to fix rat testes but plenty of protocol variations. I have started to fix some testes in NBF 10% for 24h but it doesn't look enough fixed. Do someone...

07 July 2019 4,721 1 View

Is possible to remove a co-author who only participated in the reasoning of a paper but who did not write or analyze anaything?

this is an ethical question. We have a conflict with a co-author who stopped its contributions, he only participated at the beginning of the project. I did all data analysis, article writing and...

04 March 2019 3,207 6 View

Extracting high frequency component from signal by using pywt in python?

I want to extract the higher frequency component from a signal composed of different frequency components by using the pywt package in python. How do I do this correctly? In order to extract the...

22 January 2019 4,940 1 View

How to break apart an intestinal epithelial organoid into several pieces?

I am growing 3D mouse intestinal epithelial organoids. I have polyclonal matrigel mounds. I want to isolate one organoid and break it apart and make several monoclonal matrigel mounds. How is the...

16 September 2018 1,484 1 View

What is the problem of using a predicted value as independent variable in a regression analysis (ex.: using the predicted LBM to predict Vo2max) ?

I know that a certain percentage of error already exists in predicting lean body mass and predicting a cardiorespiratory parameter such as Vo2max using this predicted measure only increases the...

24 July 2018 9,262 2 View

Does anyone know if there is a method for the complex CRISPR/dCas9 to become fluorescent only when it binds to the gDNA?

Hi, I saw that it is possible to target and image a single locus on the genomic DNA with a cocktail of CRISPR/dCas9 following the CasFISH method. However, the signal is quite diffuse and I want to...

15 June 2018 1,583 2 View

What are the conditions that you use in order to do immunostaining in differentiated 3T3-L1 cells?

Hello,I would like to do immunostainings on differentiated 3T3-L1 cells (adipocytes). We tried most of the "classic" protocols, fixation with either 4% PFA or cold methanol/acetone, and a lot of...

17 January 2017 8,549 3 View

How can I separate mouse brain vessels from parenchymal cells and assess purity of the fractions using immunoblotting (western blot/dot blot)?

Hi everyone, I am working on brain inflammation and I try to separate the mouse brain vessels from the parenchyma after the removal of the olfactory bulbs and the meninges. Currently I proceed to...

14 December 2016 4,163 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View