I am looking for a way to preserve arterial tissue for several months without affecting cell markers and lipid droplets. I currently store these tissues in 10% formalin but am concerned about the long term effect of formalin, especially on proteins. As I aim to digest these tissues with a cocktail of enzymes to get single cells for flow cytometry, another question I have is how do I get rid of formalin before digestion?
Thanks !
I don't think you will be able to digest formalin-fixed tissues. The way to do it is to digest first, prepare cell dissociates mildly fix them in suspension (1% PFA for 2-3 min), and store them in 4C in PBS. Generally flow is for relatively fresh samples.
You can not do these things on fixed tissues. You need to digest fresh tissue, isolate single cells and do flow following flow cytometry instructions.
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue