Kindly tell the chemical that i should use for extraction of great amount of secondary metabolite and can you suggest the protocol for characterization as well.
@Mahek
Hi .. hope these articles help you espicially the first one
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042905/
extraction with water, n-hexane, ethanol and methanol,....;
Headspace technique, Soxhlet, NMR, chromatography (HPLC, combined method of GC-MS and LC-MS),...
First set a target, what kind of compounds you are looking for, either polar or non-polar. Use acetone or ethyl acetate for extracting colored compounds. Polar solvents like methanol, butanol, acetonitrile, etc. are used for polar compounds like alkaloids, saponins, phenolics, etc. Hexane or petroleum ether or chloroform or their gradient solutions are used for extracting essential oil or fat groups.
Dear Mahek Sharan.
The specific details of the protocol and the chemicals used will depend on the characteristics of your fungi and the secondary metabolite you're aiming to extract. Common solvents include methanol, ethanol, ethyl acetate, hexane, dichloromethane, or mixtures of these depending on the nature and polarity of target compounds.
It's recommended to consult existing literatures, experienced researchers in the topic or a mentor in your field to tailor the process to your specific needs.
Regards
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