I have the problem with over lapping PCR can you help me please?

23 January 2024 1 8K Report

I am working on overlapping PCR with Left, EGFP and Right flank where same procedure for the primer design of the Left, Right and EGFP was using. But there is Amplification of the Left and EGFP but not the right one. from your experience i need yours advise

Jonathan Jonathan

If you can elaborate more about the problem, it would be great if you give us schematic or diagram of what you are doing and where the problem is

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