I have some house keeping genes a part of these genes have been sequenced for MLST,how I can do gene assembly?

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Antonis Tsintarakis

Dear Ahang,

I don't know if you have already found any options for your MLST analysis but you might find useful information in the following links:

https://bioinformaticshome.com/tools/wga/wga.html#gsc.tab=0

and

https://www.applied-maths.com/tutorial/mlst-analysis-starting-whole-genome-sequences

I hope this information helps you for the gene assembly,

King regards

Alexey Rakov

If you are looking for a free software, you can try UGENE or BioEdit. Otherwise, I recommend SeqMan from Lasergene.