I know nutritional cues regulate the LR emergence process, so I know it's definitely something to do with the ratios changing in the quarter strength media. Could someone direct me to a reference that has done the same or gives an explanation for the phenomenon?
Hi Rahul,
Full-strength MS is generally very rich and plants do not produce much roots in this medium. This is why people reduce the strength to induce rooting after initiating shoots, or after propagating plants. Another general tendency is to grow on a combination of cytokinin/auxin and then remove those to initiate roots or to supplement media with a low concentration of auxin.
I believe full MS is too rich for your species and once you remove that mineral 'stress' the plants grow 'normally', with lateral root formation. I have attached two papers where we induce shoots on full strength media and then root the shoots on half strength media.
I guess there won't be any references to the exact combination you use because of two reasons:
1) One percent sucrose is rarely used. 2 - 3% is what you will find in many publications.
2) Changing from full strength MS to 1/4th strength is drastic and people usually reduce the concentrations more gradually - from full strength to half strength. When people find MS too rich they tend to go for other less rich media.
Good luck!
This is because 1/4 strength provide low nitrates higher conc of nitrates are inhibitory for root development
Half strength MS with 3% sucrose and with PGR at low concentrations are said to be inducing rhizogenesis in several plant species. If it is induced in 1/4 MS in the plant concerned, it may be the requirement in vitro and also a finding.
Hi Rahul,
Please refer to this attached paper (2012): Quantitative Analysis of Lateral Root Development: Pitfalls and How to Avoid Them.
In the paragraph of "THE BASICS: THE IMPORTANCE OF GROWTH CONDITIONS", it discusses why full-strength (1x) MS medium was found to inhibit Arabidopsis root growth (Dubrovsky et al., 2009). And one of the reasons is: "The full-strength medium designed for tissue culture contains very high and non-physiological concentrations of mineral nutrients: For example, the N supply in full-strength MS consists of 21 mM NH4+ and 40 mM NO3 - ". There are also some references cited which may be useful to you.
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