You may be able to precipitate the virus at 20-25,000xg by adding PEG+NaCl quite well.
Hi,
Ultracentrifuge makes possible to centrifuge up to 170 000 g.
I used it during an internship for Stable Isotope Probing.
I hope it will help you.
There are often alternative methods to achieve the target. However, unless you elaborate what is it that you are trying to do, it will be nearly impossible for anyone to suggest alternatives.
Hi tausif , actually I am working on HIV monitoring so , i need to detect low copy viral RNA even < 40 copies that's why
You may be able to precipitate the virus at 20-25,000xg by adding PEG+NaCl quite well.
There is a very simple but useful principle: if you need some exceptional parameters of equipment, just think about the design of the experiment, sometimes there are few other solutions with same result without any very special equipment :)
For example, you can try to use membrane concentrators or dialysis technique.
Good luck!
You may even try magnetic bead based RNA isolation; using kits like Promega; RNA isolation can be done from large volume of plasma (like 1 ml) without actually concentrating the virus.
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