Is there any method to measure the recent thymic emigrants other than TREC analysis?

More Boobalan J's questions See All

I have centrifuation facility only upto 25000g but I need to use 120000g for SCA ., Any alternatives?

RNA, Molecular Biology, virology

07 August 2016 4,224 8 View

Hello all, Can Anyone explain me what is Meta analysis?

Statistics Molecular Biology Bio informatics

06 July 2016 3,894 0 View

How to use max Ratio for quality checking purposes of realtime pcr assay?

Realtime pcr, qpcr

05 June 2016 8,331 0 View

How to use max Ratio for quality checking purposes of realtime pcr assay?

Realtime pcr, qpcr

05 June 2016 4,440 0 View

Any tips on the amplification low HIV viral RNA for Sequencing?

Molecular Biology Genetics HIV Virology

11 December 2015 5,761 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Good day!! All, can anyone recommend the best battery related journals to download research articles please?

I am an Mphil research student and looking for high impact factor battery related journals to download and read research articles to improve my knowledge and get new ideas in the field of battery...

01 August 2024 6,516 2 View

Lisa Danae Schultz Johnson

If this is for mice, you could try using flow cytometry to stain for CD24 and Qa2 in the periphery. RTE will be CD24 low and Qa2hi. Pamela Fink published a recent review that may be helpful:The Biology of Recent Thymic Emigrants

Annual Review of Immunology

Vol. 31: 31-50 (Volume publication date March 2013)

Boobalan J

Thanks for your reply Lisa,

It is for humans, please send me your suggestions.

Adrian Liston

MRI for the actual size of the thymus? It correlates quite well with thymic activity

Otherwise gate on CD31+CCR7+CD62Lhi