What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

Does Abaqus plugin AM-Modeler predict the same temperature/crystallinity history as UMATHT user-subroutine?

During AM process, progressively activation of elements can predict the temperature/crystallinity history, including surroundings heat losses and than this data could be used to determine the...

06 February 2021 4,686 1 View

Are the NTD ( N- Terminal Domain) targeted Antibodies Protected from SARS-CoV-II Infection?

20 January 2021 8,052 5 View

How is it that Ad26.COV2.S Covid-19 Vaccine is Bearing Better Results?

A candidate vaccine spurs both younger and older people to make antibodies against the coronavirus SARS-CoV-2, according to early trial results. The vaccine, which is under development by Johnson...

14 January 2021 8,184 6 View

How Does a Mutation Undercut The Human Immune System to the COVID-19 Virus?

A handful of mutations to SARS-CoV-2 can help it to escape the immune response mounted by a subset of infected people. https://www.nature.com/articles/d41586-020-00502-w Comprehensive mapping of...

13 January 2021 9,545 3 View

How do we calculate curvature value in ArcGIS?

Hello everyone, I am trying to prepare curvature map in ArcGIS 10.4, using spatial analyst tool. I am using 10 m CartoDem (projected in UTM 44N). I am getting very different value, curvature...

12 January 2021 504 4 View

For How Long do the Antibodies Protect from a Reinfection of Covid-19?

The presence of SARS-CoV-2 antibodies confers subsequent immunity in most people for at least six months, a study of healthcare workers in Oxford, UK, has...

31 December 2020 8,973 6 View

How do mRNA vaccines work and what differentiates the technology from the rest?

Most vaccines contain an infectious pathogen or a part of it, but mRNA vaccines deliver the genetic instructions for our cells to make viral or bacterial proteins themselves. Our immune system...

25 December 2020 3,982 4 View

How to rectify the miscomputation of Pressure and Continuity in HVAC CFD Simulation Using OpenFOAM?

I'm using OpenFOAM to run a CFD study of thermal comfort within a hall. I'm using OpenFOAM v1912 and BouyantBoussinesq(Simple&Pimple)Foam solver. eI have 6 inlets and outlets each with specific...

21 December 2020 1,303 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View