What is your method of detection? From literature I find that methanolic extracts of C. igneus leaves contain a large number of phytochemicals such as carbohydrates, triterpenoids, proteins, alkaloids, tannins, saponins, and flavonoids .. Have you already done some literature search to narrow down what those 3 major compounds could be?
Without standards naming the compounds will be difficult. Otherwise get your HPLC coupled to a MS, it could name the compound.
As u may know, there are always range of compounds in herbal extracts. You really need the standard for accurate conclusion. If the standard is not accessible to u, perhaps u analyse the extract by NMR spectroscopy to define the compounds in the sample. Then accordingly u estimate them by HPLC base on their reported retention times.
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