I have a doubt. Is reverse transcriptase exclusive to viruses of the family retroviridae?

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How do I measure the virulence intensity of a fungus strain in a host plant?

I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...

06 May 2024 8,974 5 View

In flow cytometry, what happens if I excite a fluorophore at a wavelength below its peak excitation wavelength?

I have a fluorescent probe that I'd like to test using flow cytometry, so that I can quantify its relative binding to different cell types. Unfortunately, the flow cytometer at my institution does...

02 May 2024 241 3 View

How to keep silicone on mouse skin in a wound healing model?

Dear scientists, we are currently conducting a mouse model for wound healing. The process is as follows: make the wound, apply and suture the silicone splint, apply the tegaderm and apply the...

05 April 2024 4,776 1 View

Can you dissect tissue for RNA extraction that was previously stored in RNAlater?

I have whole insects in RNAlater but I will only use some body parts for the RNA extraction.

20 February 2024 6,537 0 View

How can laboratories improve antibiotic selections for uncommon but potentially fatal pathogens?

Providers should make an effort to understand local resistance patterns including those of the referring hospitals. Such data are relevant to monitor longitudinal resistance trends and formulate...

18 February 2024 933 1 View

Factors influencing consumer perception regarding cosmetics, questionnaires?

i need to find an existing study with survey questions

20 January 2024 2,571 0 View

Doing research in teaching Visual Thinking Strategies?

I'm a secondary school teacher who is starting a new research in the field of Visual Thinking Strategies (VTS) in Ibi, Spain. We are a group of 4 teachers that have been working VTS in our...

09 January 2024 4,373 1 View

Is constant power needed for denaturing Urea PAGE or will constant voltage work as well?

I'm attempting to separate two DNA oligos of length 120bp and 100bp. I've been running them with 2x formamide loading buffer for 3 hours at 65V. I understand constant power is important for the...

28 December 2023 5,457 2 View

Gastroplus - Determine D90 of log normally distributed particle size population?

For gastroplus modeling - can I determine the D90 from the specified D50 and SD/bins from the particle entry section? I've modeled several scenarios using a specified D50 and SD and # bins, but...

11 September 2023 9,204 1 View

How can I characterize the dark spots (probably melanocytic tumors) in drosophila melanogaster?

Hello Everyone, I'm a master's student and I'm currently working with drosophila melanogaster mutants. Right now I have a mutant that has dark spots in various regions of the fly's body (in the...

03 September 2023 7,795 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View