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Questions related from B R Siddharth
As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...
12 July 2024 4,255 0 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
12 July 2024 8,956 3 View
phenol chloroform extraction method is used for purification of nucleic acids DNA/RNA
04 April 2024 8,223 0 View
phenol chloroform extraction method is used to purify nucleic acids to obtain a good concentration.
04 April 2024 7,202 0 View
as viruses are notorious for being prone to mutation at high rates its very difficult to clone them though general cloning procedures and cells so in order to solve this problem need specific cell...
04 April 2024 8,990 5 View
BAC vectors are use for cloning purpose and are good for cloning large fragments though they have low copy numbers.
26 May 2023 5,108 2 View
reverse genetics platform is used for cloning platform where cDNA is made from Viral RNA and is used for cloning
15 February 2023 4,623 0 View
durring reverse genetics platform RNA isolation is a necessary step and from there we proceed for cloning
15 February 2023 5,463 2 View
transformation after ligation is nessesary step to get desired product in large amount.
04 February 2023 2,882 4 View
after bacterial transformation bacteria need to be speeded on a percitiplate to do so we need to pallet bacterial cell and it is subjected to centrifugation.
03 February 2023 2,814 8 View
cloning and trasnformation of bacteria containnig ampicilin resistant gene
16 January 2023 5,731 4 View
loading dye to track DNA smaples on electrophoresis and load them on wells
07 October 2022 8,012 3 View
for synthesis of nanoparticles, a sharp peak must be obtained around 410-450nm
10 December 2019 5,339 5 View
chromosomal abnormalities induced due to biological silver nano particles in plants
03 October 2019 4,321 0 View
We have attempted to synthesize Silver (SNPs) from Fenugreek any suggestions regarding improvement and how to interpenetrate this graphs we have used 3mM Silver nitrate and 30% plant extract.
25 September 2019 9,747 1 View
Surface plasmon resonance of silver nano particles synthesis
25 September 2019 4,972 5 View
root tips are used to evaluate genotoxicity caused due to different agents like silver nano and industrial effluents.
23 August 2019 9,106 0 View
SPR indicates formation of silver nao in UV-vis spectrophotometer data it gives SPR Peak
19 August 2019 5,993 4 View
Uv- vis spectro reading in 300-700nm range for silver nano sunthesis
16 July 2019 1,926 14 View
to study effects of silver nano particles on plant Genotoxicity
13 June 2019 229 1 View
silver nano particles create chromosomal aberrations in plants
10 June 2019 3,516 2 View
silvernano particles are produed from palnt extract various chemical and physical process can be used to produce silver nano particles
05 June 2019 6,948 5 View
microgrens are used as a food source from plants
05 June 2019 1,983 2 View
This is an open thought question for future xenobotanists. I read that NASA is hiring a new round of astronauts for their Mars project, and it made me think about botany that is not on...
01 January 1970 3,092 3 View
optimization of Ag NP synthesis
01 January 1970 7,752 1 View
genotoxicity at chromosomal level
01 January 1970 8,674 0 View
Nano particle characterization
01 January 1970 5,502 6 View