I got reddish DNA pellet after final washing steps, Should DNA extraction Washing steps be repeated if the desired DNA contains TE Buffer in it?

More Sidra Basharat's questions See All

Is there any free Journal to publish systematic literature review protocol? I have checked JMIR but they are also charging for protocols?

I am looking for a free Journal (good impact factor) to publish a systematic literature review protocol. I have checked a few but they are charging a lot. Any recommendation will be appreciated....

19 November 2023 9,285 2 View

How we do sequence analysis for insert?

What does it mean sequence in frame. How we do full read of sequence.

16 October 2023 4,139 1 View

Suggest the method for preparation of MoS2 nanosheets?

I've tried the liquid exfoliation method but I'm not getting the results. If anyone else has tried through this method can anyone share protocols so that I can check where I'm making mistakes in...

10 September 2023 3,753 0 View

If I have scale dependent variable and ordinal independent variable, what can be the possible options of tests for research in SPSS?

Can I do regression analysis for such kind of variables?

02 August 2023 3,619 3 View

How can I Synthesize 1-bromo-2-hexanol in good yield? and how to purify the reaction mixture?

I am following this protocol (attached) but I get more of side product (bromo hexane) and very less amount of 1-bromo-2-hexanol.

07 June 2023 6,169 0 View

In linear response formalism, is it necessary to use a supercell for phonon dispersion of Perovskite structured materials?

Normally in finite difference method, a large supercell gives good results for phonon dispersion. However, for linear response method, the primitive cell can be used.

31 July 2022 786 1 View

SDS gel bands resolution is not satisfactory?

I have change all the buffer and also replaced aps and acrylamide still not getting good resolution of bands and gels is smeared from the bottom.My protein size is 23 kd. Kindly provide your...

22 April 2022 4,353 3 View

What is an alternative test for chi square on frequency data?

Is there any other statistical test which, can be applied in the frequency set of data for figuring out the statistical significance?

11 December 2021 1,356 3 View

When we apply PCA to construct an index, what should be commands procedure?whats the difference of new variable with roation and without rotation?

I want to construct an index through PCA, i applied rotate command before predicting the new variable. Should we rotate or not?answers will be different or not? and which is better way to apply PCA?

14 November 2021 3,253 0 View

Can I transform my previously published paper into a book chapter?

What is the worth of publishing a book chapter?

07 August 2021 468 12 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View