Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

How do I correct an inaccurate citation?

I was asked to verify a citation: Drugs and galencials, by D. C. Garratt, B.Sc., Ph.D (Lond.),... The author's first name is Emerson and the last name is Beeler. It was incorrectly cited as...

03 February 2021 5,272 3 View

Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

20 January 2021 9,019 1 View

I have a challenge with a structure I optimized it's weight but the frequency constraint is not satisfied?

I know that frequency depends on material properties, geometry, support conditions and in plane loading. I have played around with these figures except that I maintained the same material steel...

26 December 2020 4,877 9 View

How can I coat reduced graphene oxide on glass substrate?

I dispersed reduced graphene oxide in NMP solution and tried to coat it on a glass substrate using dip coating system. But I am not able to get a uniform coating. Please suggest me a solvent and a...

26 November 2020 8,929 4 View

How can I measure the sheet resistivity of my reduced graphene oxide thin film?

I tried to coat my NMP dispersed rGO sample on the glass substrate by dip coating system. Then I characterized it with four probe system for sheet resistivity measurement. But I got a very high...

16 October 2020 3,696 3 View

Heat and plastic in MTBE lipid extraction?

When extracting lipids with the MTBE method, the indicated temperature is RT. In addition, drying the samples in a speedvac is mandatory due to the presence of water and such procedure is long and...

14 October 2020 9,776 2 View

Does anybody have an idea of how to solubilize hydrophobic peptides?

My hydrophobic peptide (12 AA) is soluble in 100% DMSO. Given that I should encapsulate it in hyaluronic acid nanoparticles, I need that peptide is soluble or partly soluble in water. Does someone...

03 March 2020 8,025 4 View

How do I choose which EEG trials to run in Common Space Pattern (CSP)?

I am currently working on a binary classification of EEG recordings and I came across with CSP. As far as I understand, CSP allows you to choose the best features by maximizing the variance...

20 January 2020 7,536 2 View

For researchers who have a P-97 Micro-pipette puller from Sutter instruments, can you help me get the values of factory pre-programmed Program #1?

We have lost the parameters of this program by mistake in our lab. And we need to reenter those, to our new P-97 puller. A picture would be good. I appreciate the help.

15 October 2019 5,710 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View