Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How do I change a preprint to published?

I want to know how to change the status of an article.

21 February 2021 1,536 2 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

What can be the Research Design if I am looking for Correlation Coefficient and Regression Coefficient?

In our research our title includes the impact of academic anxiety on academic performance. This one is a quantitative research. Our data is numeric. Can someone suggest what can be the research...

21 January 2021 1,650 3 View

What would cause Cell Titre Glo assay wells to luminesce less than the media only wells?

Hi all, I'm doing a bunch of Cell Titre Glo assays to assess the effects of several inhibitors on 2 cell lines. Problem is, for some of the wells they seem to be luminescing less than the...

30 October 2020 1,513 2 View

Can someone give me an example for a questionnaire regarding roger's innovations in organizations?

Hello, I am looking for a questionnaire based on Roger's Diffusion of Innovations - Innovations in Organizations theory. Can someone give me an example?

15 September 2020 8,351 4 View

Are there studies on fallback fruits of avian frugivores?

Hello everybody! Fallback fruits are fruits of low nutritional value that in periods of fruit scarcity become the filler or staple of a frugivorous animal. Fallbacks seem to be well-studied in...

17 August 2020 7,923 4 View

Can I get some help and reading these results? I ran a regression analysis of students pre and post test grades?

After conducting a linear regression analysis of Interpretive Reading (Pre-test) scores (Table 12), the coefficient or Y-intercept associated with the pre-test scores, (0.696985136) is zero, and...

02 July 2020 7,309 1 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View