I d like to add my work on RG. How to do it?

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PVP can be dissolve in 1- pentanol or 1- butanol? It's the same result?

at the preparation of the C18 alkyl silica, the paper used n-pentanol to dissolve PVP, in my lab I have 1- butanol. Could it be the same?

20 May 2024 5,933 1 View

What are absorbance maximum and extinction coefficient of 4,4′-Diisothiocyano-2,2′-stilbenedisulfonic acid?

Maybe there are more fresh data than old reference in Sigma-Aldrich?

19 May 2024 9,483 0 View

¿Conocen software o apps que recomienden para la transcripción de grupos focales o entrevistas grupales en español?

Hola a todos/as! ¿Cómo estás? He visto que existen apps con AI para asistir en la transcripción de grupos focales. Probé uno (Notta) que tiene una versión no paga que alcanza para testearlo y no...

17 April 2024 640 0 View

How could we implement -xthenreg (GMM threshold) with robust errors (Stata14)?

Could you please clarify how to add robust (white) errors to the function -xthenreg in Stata14? I am running Threshold GMM Estimator (Seo and Shin (2016)) for my panel (N=60, T=28). Commands such...

03 April 2024 8,500 1 View

Where can I find floral sleeves for 10cm x 10cm pots in the UK?

We use Aracons for Arabidopsis but need something to contain larger plants in larger pots. All garden supplies stores I've been in person and online only sell sleeves with 10cm bottom openings,...

02 April 2024 9,337 0 View

Which cell-penetrating peptides (CPPs) do you recommend to deliver intra-cellular cargo in chitin cell-walled unicellular eukaryotes?

Dear colleagues, I am working on a project in which I want to deliver oligonucleotides (specifically, molecular beacons (MBs)) inside different protist species. To do so, I am going to use...

18 March 2024 2,778 0 View

¿Estarían formulados correctamente los siguientes objetivos para abordar una adecuada intervención con víctimas y supervivientes de trata?

1.1.- Reducir los obstáculos de las personas víctimas o posibles víctimas de trata con fines de explotación sexual y sus hijos/as en su acceso a recursos, prestaciones y ayudas forales. 1.2.-...

10 March 2024 8,166 1 View

Is there any posibility that the phase matching of gcpw is depended on spacing or anyother factor?

I am designing two RF tracks (Transmission lines) based on Grounded Coplanar waveguide. The length of both tracks is same, but there is still a phase difference between two tracks.

16 February 2024 6,718 2 View

Which tool is the most reliable for predicting RNA secondary structure in oligonucleotides?

Dear colleagues, I am designing RNA molecular probes for FISH, and I want them to adopt a hairpin structure. I have checked for the possible secondary structure formation of some of the candidate...

14 February 2024 7,811 2 View

If i delete my google sholar profile, can i create a new one?

If i delete my google sholar profile, and 30 days for recovery profile pass, can i create a new one?

14 December 2023 8,329 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View