Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

MHC Rabbit Genome Annotation? Domain annotation of each MHC Rabbit Gene?

I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...

28 January 2021 7,722 2 View

Has anyone found a way to reliably stain microglia in postmortem frozen brain tissue?

Antibody based approaches aren't working as well as for other cell types, even IBA1 isn't working that well. Does anyone know of a histological/chromogenic stain for microglia? Or any other way?...

13 September 2020 5,927 3 View

Tips on reconstitution of lyophilized material with a small volume?

Has anyone got any advice on how to reconstitute lyophilized material, contained in a 3mL glass vial, through an igloo cap, with only 125uL dilution buffer? I find that the volume is so small that...

21 August 2020 4,347 3 View

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro (laboratory)culture?

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro culture? I am looking to replace the Provasoli medium with something simpler and cheaper (I have...

10 August 2020 302 3 View

What is statistical meaning of testing in machine learning?

Machine learning model, normally, is applied on new data for testing. If the accuracy of the model is below a certain threshold, the model is rejected. But why? This is just a random sample, the...

25 July 2020 8,615 4 View

Why Autodock Vina gives me an error when it is writing the output file?

Good afternoon, I was a performing a docking trough Autodock Vina, following a tutorial from the Scripps. I sent the corresponding commands to run the docking using a configuration file that I...

15 March 2020 2,328 2 View

Could someone help me with my PCR reaction for genotyping?

I used to genotype my KO animals following this protocol: I extracted the DNA from the tail using a Sigma reagent and I performed the PCR reaction with a Promega Green MIX. It worked well, but due...

11 March 2020 7,926 1 View

Is it correct to consider EEMS (Petkova et al 2016) results when observed and fitted dissimilarities are not correlated?

I am using EEMS to analyze a sample of rodents (Calomys venustus) genotyped at microsatellite loci, living in an agricultural landscape. According to diagnostic scatterplots provided by EEMS, data...

04 March 2020 4,411 2 View

What can be the best method of estimating the air quality in Piraeus Port in Athens?

I want to conduct this research in the summer months, in order to estimate if high temperature and high air pollution are strongly correlated and if temperature is an estimator of the pollution....

09 February 2020 5,512 7 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

Which photsynthetic machine is good to use for measruing photysnthesis and CO2 assimilation for Arabidopsis?

Hi all, we are looking to buy a photosynthesis meter that can potentially measure photosynthetic rate, stomatal conductance, CO2 assimilation, PS1 and PSII efficiency (if possible), etc. it would...

02 February 2021 5,223 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View

Equation to calculate Chlorophylls and carotenoids concentrations from a MeOH:HCl 1% (v/v) plant extract solution?

Hello everybody! I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on: Rabino, I., and Mancinelli, A. L. 1986....

25 January 2021 3,686 2 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...

23 January 2021 2,790 3 View

Can use Unigenes from RNA sequences for overexpression studies (transformation) without requiring a specific plant genome?

Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...

20 January 2021 5,557 2 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View