I am unable to regenerate plants from the callus obtained from apical meristems. Also, the callus response is not defined and changes frequently and is not replicating. The variety produces a lot of phenolics and before they reach regeneration , they die. I find it hard to decide whether to go for calli that are compact and hard or calli that are friable and wet. The wet ones do not survive on the regenertion (shoot) medium whereas compact ones produce rooting response on the regeneration (shoot) media. Phenolics are a big problem and the type of callus also. Please suggest solutions for troubleshooting the media composition.
charcoal scanvenges or sequesters all the hormones and affects the callus growth. Is there any other means to exclude tannins and phenolics secreted by the plant without sequestration of hormones and additives?
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