Sakshi Mehta

5 Questions 11 Answers 0 Followers

Questions related from Sakshi Mehta

Trouble in amplifying a particular gene?

I have trouble in amplifying a gene. I am continuously trying to find a possible solution. All methods have failed me somehow. I was amplifying gene A using gene specific primers. This gene has a...

22 May 2018 5,030 8 View

Can anybody explain or compare the difference between callus and tumor?

Generally callus is undifferentiated mass of cells, Whereas tumor is uncontrolled growth of cells, some say both are comparable, but I disagree, any idea Note: I am talking from a plant biologist...

22 May 2018 8,545 3 View

How to make sure if your RNA has been converted to cDNA?

I am trying to amplify a gene from cDNA. The gene contains an intron, therefore, I am amplifying it from cDNA. When I amplified it using genomic DNA, it shows the desired band. But when I am...

05 April 2018 3,302 8 View

How to reduce degradation in Agrobacterium RNA ?

I am isolating RNA of Agrobacterium and when I performed it for the first time it showed no degradation and now it is showing degradation. What can be the possible cause? I used Trizol method...

13 February 2018 4,922 5 View

I am working on a tannin variety of a monocot that produces a lot of phenolics. I have problem in regenerating plants from the callus. suggestions?

I am unable to regenerate plants from the callus obtained from apical meristems. Also, the callus response is not defined and changes frequently and is not replicating. The variety produces a lot...

02 December 2017 1,498 1 View