When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Are there any mice that express rtTA (Tet-on) in the brain and spinal cord when given Dox?

I am looking for a Tet-On Tg mouse in which rtTA is expressed in the brain and spinal? I cannot seem to find any a JAX Labs and I wonder why? All comments welcome.

29 August 2020 766 1 View

What is the alternative method to centrifugation to remove acid residues from sediments during carbonate removal process?

I am removing carbonates from clay and sand samples. So, I treat the sediments with H2O2. I need to wash the sediments to remove acid residues. I am thinking of heating the sediments with...

06 December 2019 5,057 6 View

Why are the background values hampering when mosaicking bulk images?

Hi all!! we have been encountering the following issues while mosaicking multispectral data: while mosaicking 4-5 or more scenes/tiles, the background values are either 0 or 255 of the mosaicked...

26 August 2019 5,496 3 View

What column should I use to analyse CO2 reduction products?

Hi, I currently have a HP 6890 GC equipped with an FID and DB-624 column. I am upgrading the instrument with a gas sampling loop and a TCD detector. I plan to use the FID to analyse short chain...

02 March 2019 9,871 2 View

Can we consider 14 dB axial ratio as linear polarization for practical purposes? If not , what is the standard norm if any for practical purposes ??

Theoretically the axial ratio of linearly polarized antenna is infinity. But practically it is not possible. So, is there a practical/industrial norm for differentiating elliptically polarized...

26 February 2019 9,438 5 View

For LoS communication the transmit and receive antennas face each other. If the transmit antenna is vertically polarized,,what about receiver antenna?

30 January 2019 989 3 View

How to obtain Clay % from Fine content (silt+clay) % using empirical formula?

Dear all, I have calculated Fine content (Silt+Clay), Liquid Limit, Plastic Limit, Plasticity Index, Mean grain size, SPT value for soil samples. I need to obtain Clay % for my study. Kindly...

13 January 2018 2,502 8 View

How to prepare TfOH, HBr or HCl salt of BINOL derived phosphoramide containing one pyridinium ring?

I want to protonate the pyridinium nitrogen (BINOL derived phosphoramide containing pyridine ring) to prepare a salt. I do the reaction in dry DCM at room temperature using TfOH, HCl in Dioxane (4...

01 August 2017 3,149 3 View

Have others experienced a reluctance on the part of publishers to investigate reviewers' unethical behaviour?

We recently received an offensive review which accused us of plagiarism, dishonesty and fabricating observations. Three editors considered this a fair review, and a representative of the publisher...

28 June 2017 6,236 9 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View