Just curious if there is so much endogenous GST within cells, why would you use a GST-tagged recombinant antibody and surely you will purify/detect interfering endogenous enzyme when using this system? The sequence of the GST Tag and the GST (pi isoform) found in most cancer cells is 80% similar according to a BLAST of the sequences. Any light anyone could shed on this would be much appreciated. Thank you
If you are asking about using an anti-GST antibody to immunopurify or detect a GST-tagged protein from cells, my guess is that the epitopes of the monoclonal antibodies have been selected so that they recognize the GST tag and not the endogenous GST. Since the epitope of each monoclonal antibody consist of a small number of amino acids, it should not be too difficult to develop such a selective antibody.
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