I am looking to culture neonatal cardiomyocytes (both atrial and ventricular) and I am curious if anyone has had success doing this using poly-D-coated dishes. Thank you!
Research being conducted by Stellenbosch University would like your opinion about the effects of social exclusion on emerging urban mobility options and traditional public transport services. By...
21 July 2024 4,032 0 View
I will be administering 5-fluorouracil at a dosage of 50 mg/kg to mice as an I/P injection. Will someone please check my calculation? (Mw: 130.078 g/mol) Max volume to be administered to mice I/P...
23 June 2024 3,847 1 View
Dear Survey Experts, I am currently researching trust and resilience in the supply chain and seeking efficient ways to find survey respondents. In addition to social media and business...
21 June 2024 395 2 View
Hi! I have a question. I was looking into the research question: does mohs micrographic surgery give less recurrences and metastasis than standard excision in the treatment of cuteanous squamous...
17 June 2024 7,769 5 View
I have porous PMMA microparticles up to 1mm in diameter. I would like to functionalise the surface with amines whilst keeping the particles intact. Chat GTP has recommended ethanolamine or ATPES...
13 June 2024 7,977 1 View
Hi, I have been using a Q-Sonica Q500 Sonicator to disperse samples on nanocellulose and make emulsions. I have notices black dust like particulates settling out in some samples and on the bottom...
12 June 2024 9,737 6 View
I'm working on the RAFT copolymerization of MPC and NIPAm monomer, but I'm not sure how to calculate the total conversion from proton NMR? Is it an average of conversions of two monomers or just...
10 June 2024 7,430 2 View
Dear ResearchGate, I came across the ResearchGate page of Adri Jacobs, who passed away in 2010. This page contains 25 publication of Adri mainly from the period 2000-2008. One paper (2011) must...
01 June 2024 8,763 1 View
I am looking for resources in the Netherlands regarding cultural tourism. So what cultural activities correlate, and what lifestyles can be distinguished. Any resource is welcome, articles, factor...
27 May 2024 447 2 View
I've coated plastic water bottles (PET) with Nile Red to detect the plastic degradation by Ideonella sakaiensis. When using the settings for Sypro Ruby, the coated and uncoated plastic looks the...
23 May 2024 3,364 0 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...
20 June 2024 393 0 View
I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...
20 June 2024 3,225 2 View
Maintaining a WiCell fibroblast-derived line of iPSC in monolayer with laminin coating and E8 media, then using Wnti directed protocol (Gsk3i day 0, Wnti with conditioned media day 3, insulin day...
02 May 2024 6,314 1 View
I have tried isolating ventricular cardiomyocyte by perfusing type II collagenase through adult mice hearts. When looking at the extracted cells under 20X magnification, I don't see the typical...
21 March 2024 3,725 1 View
I conducted the recovery from inactivation protocol to record INa in isolated cardiomyocytes. The very strange thing is that the sodium current couldn't return to the max value (marked 1). The...
14 March 2024 9,544 14 View
What experiments can be conducted to compare the proliferation ability of human induced pluripotent stem cell-derived cardiomyocytes (hiPSCCMs) with a specific gene knocked out using CRISPR-Cas9...
31 January 2024 1,373 4 View
We are looking for a pulse protocol with a prepulse which could eliminate Na+ and K+ currents
23 November 2023 9,833 0 View
Hi! I do patch-clamp on cardiomyocytes in whole-cell configuration, voltage-clamp mode. Everything went well but suddenly the baseline started to drift down (when the electrode is in the bath,...
22 November 2023 4,376 0 View
What is the best cell line for Cardiomyocytes for in vitro functional assay. I need a robust cell line easy to transduce and maintenance. I have several candidate to consider: C21C2, AC16, and...
12 November 2023 2,144 3 View