I am looking to culture neonatal cardiomyocytes (both atrial and ventricular) and I am curious if anyone has had success doing this using poly-D-coated dishes. Thank you!
I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...
11 February 2021 2,416 2 View
Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....
14 January 2021 6,326 2 View
Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...
06 January 2021 7,247 3 View
Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...
03 January 2021 1,010 6 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
27 December 2020 3,688 3 View
Many Universities have employees located across the world.
22 December 2020 6,380 3 View
I have been making a method to determine chlorhexidine using an LC-MSMS (ESI+ modus). My solutions are made in acetonitril/water (65/35) and 0,2% formic acid is added to ionize the compound. My...
14 December 2020 3,855 6 View
Is it true that only a very large sample is suitable for a panel data fixed effects estimation, or do the ols criteria apply?
26 November 2020 9,127 4 View
Hello all, Early next year I'm planning to evaluate a training for school principals. I will collect data from school principals with experience (what that entails is to be defined, but they...
13 November 2020 2,226 4 View
Hey, I am conducting an event study with a short event window (lets say 3 or 5 days). I have a lot of variables, but most of them are fixed over that short period of time (GDP per capita, GDP...
11 November 2020 3,582 6 View
I have faced a problem with culturing Ac16 cardiomyocytes cell line. Day 0 I plated 200,000 cells/2ml (well) in 6 wells plate in FGM. Day 3 I put my cells on Low serum medium. When I changed...
24 January 2021 1,456 2 View
I have GD18.5 heart tissue currently snap frozen in -80, apart from mRNA and protein analysis on whole tissue, is there a way of isolating single cell populations to study localization of a...
19 January 2021 4,044 3 View
Hello, I followed a protocol for isolating human cardiac stem cells (Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human...
18 November 2020 2,928 3 View
From the literature I understand that TRP channels regulate Ca2+ signalling in cardiomyocytes and fibroblasts. I want to perform immunocytochemical analysis of TRP channels on cultured cells or...
07 May 2020 6,865 1 View
BCG vaccine is being seen as a new hope for COVID-19 patients. It can make our immune system to recognize and respond to a variety of infections, including viruses, bacteria and parasites etc...
27 April 2020 7,728 30 View
Hello everyone, Which is the best way to resuspend a culture of adult rat ventricular cardiomyocytes without damaging the cells integrity? This is what I am trying to do. I isolate ventricular...
31 December 2019 1,080 2 View
Hi everyone, We have difficulty sectioning neonatal brain tissue. We are following this protocol: We dissect the whole brain and use a 4% PFA fixation until several days. We do not perfuse the...
31 December 2019 6,679 4 View
I would like to know if anyone has a suggestion on how to induce, around postnatal day 10 (P10), an activation of OXT or AVP neurons in mice pups ? A stressor? some handling? a separation/reunion...
11 December 2019 1,344 2 View
I have been trying to culture primary cardiomyocytes from neonatal Rat heart using PIERCE Cardiomyocyte isolation kit. I have plated the cells 4 days back and changed the medium after 48 hours....
08 September 2019 8,216 3 View
I am trying to label phospholamban with antibody 2D12 and proteinA-647. Sometimes I get the result below. I don't know what happened.
08 September 2019 9,949 5 View