We are looking for a pulse protocol with a prepulse which could eliminate Na+ and K+ currents
Dear All, I am trying to define the dispersion curves of a periodic unit cell in COMSOL Multiphysics with prescribed displacements. I am using two separate studies: - Study 1: Stationary, with...
13 July 2024 7,855 1 View
Hi all, I am conducting research on screening peptides (20-35 amino acids) with potential for treating dengue fever through protein-peptide docking methods. In the protein database, I found the...
10 July 2024 8,037 1 View
I am purifying RNAse E in E. coli (118 kDa). I cloned the rne coding sequence on pET28a with a His tag at the N-terminus. The protein overexpressed fine (see attached figures). The binding buffer...
24 June 2024 6,287 4 View
Does anyone have experience with plant meristem culture? What kind of plant growth regulator combination is best? From what I read, there seem to be 2 methods. One using cytokinin (BAP, kinetin)...
08 June 2024 7,877 2 View
i have a species trichoderma harzianum. It is development in PDA media normally. But it is producing yellow pigment on rice broken at fermentation.
31 May 2024 9,260 0 View
Hi everyone, I am attempting to replicate the results of a published paper to determine the mass-effective density in order to elucidate the bandgap formation mechanism. The paper is titled...
16 May 2024 3,272 0 View
Dear all, I have been trying to quantify phases in a powder mixture including LiMnO2 (orthorhombic, cubic, space group: Pmnm) and Mn2O3. I'm trying to use the Rietveld method, but concerning...
14 May 2024 5,788 3 View
For phylogenetic analysis using SSRs, normally we don't use just one SSR, but a set of multiple SSRs to ensure accuracy. So how can we input these data into NTSYS? I know input for one SSR is as...
13 May 2024 1,867 1 View
Hi all. I know that crystal field splitting occurs in octahedral complexes in magnetic materials. However, I'm unsure whether crystal field splitting depends on temperature. Can crystal field...
28 March 2024 452 1 View
In all academic sources, sucrose is identified as α−glucose (1-->2) β−fructose. However, I cannot find any explanation anywhere as to why the fructose monomer has to be in the β configuration....
12 March 2024 9,230 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View
Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...
01 August 2024 572 0 View