Najmi La I hope this answer helps you in your studies.

It typically depends on the type of functional analysis you are planning for your studies.

If you are looking for cell lines that exhibit contractions, iPSC-derived cardiomyocytes are a good choice. AC16 cells don't exhibit contractions, but you can easily maintain them with good proliferation.

C2C12 is a skeletal muscle cell line. Although it exhibits contractions, this behavior is different from what we usually observe in cardiomyocytes because they will exhibit contractions only after forming into myotubes (after differentiation). I am not sure about human cardiomyocytes because it typically depends on age. You may observe some cell proliferation and attachment in the neonatal stage, but adult cells behave differently, and you need to maintain them in a specific medium and stimulate them to maintain functionality.

Hi Budharaju,

Thank you for your response!

Contraction is not the essential but definitely exhibit the cardiomocyte structure and easy to transduce.

iPScells or primary dissociation of adult or newborn rodents