I am using the WST-1 assay to quantify cytotoxicty in my cells. I have a hunch that my experiment (a drug treatment) causes abnormal activity in their mitochondria. I have observed toxicity by eye in previous experiments compared to the control/untreated cells, however my WST-1 assay is suggesting that these cultures in fact have better viability than my controls. Could it be possible that the tetrazolium is being metabolised faster in these 'abnormal' mitochondria hence giving me a higher readout? I am not certain of how sensitive this assay is to the potential differences in mitochondrial activity between my samples.
Heshu Sulaiman Rahman
Pls kindly use MTT assay as it is better to determine cytotoxicity. TQ
0 votes
0 thanks
- Badges
- Science method
Similar questions and discussions