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11 January 2021 3,284 3 View

How can we count the number of edges in an edge detected image using canny/sobel algorithm in MATLAB?

I need to process an image for edge detection and then want to count the number of edges in that image.

29 December 2020 5,594 2 View

Which are the vendors who sell commercial SWIR (NIR-II) dyes?

I am searching to find commercial vendors who sell SWIR dyes. I could only find Nirmidas Biotech which sells different NIR-II dyes!

23 December 2020 5,002 3 View

How to get doi of presentation ?

doi of your presentation in the conference

23 December 2020 9,370 2 View

Precipitation of Pcl from polymeric solution ?

We working on PCL nano particles 1).Ketoprofen (10mg)+ dmso(5ml) Pcl (10mg)+ DCM(5ml) 2).Water 10 ml+ 10 mg When we mix both 1+2 after solublization (400 rpm) Why precipitation of polymer occurs?

09 December 2020 5,033 11 View

How to induce doxorubicin resistance in MCF-7 and MDA-MB-231?

I want to establish DOX/R cell line in vitro for the research study.If any one suggest the protocol then it would be a great help.

03 December 2020 9,795 5 View

What is the difference between weak from Ritz method and variational form Ritz method while solving a boundary value problem ?

What is the difference between weak from Ritz method and variational form Ritz method while solving a boundary value problem ? Can all differential equations take variational form ?

18 November 2020 2,341 2 View

What could be the reason for a broad peak of ADC(Antibody Drug Conjugates) using HIC-UV method (Hydrophobic Interaction Chromatography)?

Hi, We are working on DAR (Drug to Antibody Ratio) estimation of ADC using the following HIC-UV method. For some of the unconjugated mab we getting a sharp peak but for our desired mab we are...

15 November 2020 7,359 0 View

Can SEM-EDS give a chemical formula?

I have attached an excel file with the quantification of a mineral I conducted EDS on. I have more excel files of minerals too as I know EDS spectra for only 1 sample will certainly not be...

03 November 2020 6,899 9 View

Can TBDMS protecting group be cleaved under 25% TFA/DCM?

I performed a boc deprotection via 25% TFA/DCM (1 hr) without any workup, which should yield TFA salt as a product. My compound also had a TBDMS group on it. But when I took an NMR, the amine peak...

21 October 2020 4,470 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Mixture neutralization after Boc deprotection ?

I am performing a Boc-deprotection with HCl in organic solvent (dioxane or Ethylacetate) but would like to isolate the free amine. Several problems: - Neutralizing with inorganic base as NEt3...

18 February 2021 3,366 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View