I am supplementing ITS to culture media during in vitro culture (7 days) of oocytes.
Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...
07 July 2024 9,413 4 View
I currently run into the issue that our iPSC with a TET-O NGN2 construct seem to die from day 7 after conversion with limited cells alive by day 9. I have observed the issue in 4 individual lines...
20 March 2024 1,332 2 View
Dear all, My co-workers and I have presented a small paper on the 36th BLED conference. The paper is published in the conference proceedings (https://dk.um.si/IzpisGradiva.php?id=84589) and the...
08 October 2023 6,246 3 View
Hi everyone, I wanted to ask some advice on EPSC detection using Neuromatic in IgorPro. I'm quite new to the software and I'm struggling to detect true events, it still includes a lot of...
20 July 2023 5,200 2 View
Hello I am Anouk, 22 years old and graduated from professional bachelor biomedical laboratory technology me majoring in pharmaceutical and biological laboratory technology. I would like to switch...
02 July 2023 5,362 2 View
EPMA question: We were measuring Cassiterite samples on our Jeol FEG-EPMA (15kV, 15nA, focussed beam) - using a cassiterite primary Std to standardize Sn. On the unknowns (also cassiterite), we...
08 May 2023 5,990 4 View
Hello, I am currently busy with my masterthesis and I have a question regarding the bootstrap of the paired samples t test. My data contains a small sample size (n =18). My supervisor advised to...
04 June 2021 148 3 View
In SPSS I'm doing the analysis for PROCESS model 7, but I'm struggling how to do the write-up. Would be great if anyone could help me out :)
25 May 2021 8,897 3 View
How can I assess the SUVmean, max and ratio in a PET-CT scan using Horos? I know how to create a ROI and read the hounsfield units/Bqml for CT or PETCT scans but I don't know how I can see what...
09 May 2021 9,661 1 View
I'm working on my thesis right now about relationship between sport heritage values and supporter's sense of place (of the city they live in). I had the idea that I should first measure the...
07 April 2021 3,472 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...
01 August 2024 9,349 2 View