What is the effect on the conductivity of the thin film during the process of thermal evaporation ?

Why thermally evaporated (VCM 600 V1 configuration of the thermal evaporator) CdTe film found n-type not p-type conductivity whereas all CdTe films deposited by varying the source to substrate...

23 November 2020 7,427 3 View

Can I analyse NGS DATA from an EXCEL spreadsheet?

Hi, I have Fold expression of RNAseq data obtained from Ion Proton sequencer and got the fold change data with mean in an excel sheet. now can I analyse this fold change data using any...

20 October 2020 2,240 3 View

Monolitic construction of brick masonary and concrete simultaniously have any effect ?

Concrete column casting after masonary completed monolithic.

17 August 2020 7,172 3 View

Rentraint Corner of building if need extra stitching diagonal reinforcement ?

If temperature reinforcement already provided ,at reentraint corner need extra diagonal reinforcement ,if needed then what amount of steel.

12 August 2020 1,707 2 View

No title

26 July 2020 1,511 0 View

How to calculate Time Average data (WSS, Velocity, Pressure) in ANSYS Fluent ?

I am doing a blood flow research for which Time average data of Velocity and Wall shear stress is important. The study is transient and my flow time is 0.5 sec. I save data with time step size of...

07 July 2020 4,323 2 View

What is the best management strategy for Diamond Back Moth (DBM) in cole crops?

What should be the best managemnt option for suppressing the DBM problem that farmer can take into consideration?

11 May 2020 4,280 1 View

Is it a good idea to retract a submitted article as the journal is taking too much time to make a decision?

I had submitted a research article related to the field of mechanical engineering (CFD) on the last December, 2019 in a Scopus Indexed journal. It has been around 6 months but the journal...

11 May 2020 9,286 8 View

Where can I get details of SARS-CoV-2 infected patients?

I have been working on peptide vaccine for SARS-CoV-2 or COVID-19 and also recently published some analysis on mutational changes in this virus. It would have been really helpful if I could get...

05 May 2020 2,749 9 View

How does the chemical etching (using HF) affect the temperature sensitivity of a fiber Bragg grating (FBG) inscribed in silica fiber?

Due to chemical etching, the cladding diameter of the silica fibre decreases with time. How does this reduced cladding diameter affect the temperature sensitivity of an FBG, which is inscribed on...

17 April 2020 4,771 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View