Debkanya Dey

3 Questions 1 Answers 0 Followers

Questions related from Debkanya Dey

What is the best Taq polymerase for a low copy number gene?

I want to amplify a gene which has less copy number, Please suggest suitable Taq polymerase which do not have 3'-5' exonculease activity.

13 September 2023 4,627 1 View

What is the correct choice of DNA polymerase here?

I have been using a PCR master mix green coloured from a Promega ( green taq). But for my recent work in a particular gene that PCR master mix is not working. I borrowed Phusion DNA polymerase (...

12 September 2023 8,159 1 View

I am unable to make 3% agrose gel.What to do?

My 3% agarose gel is setting unevenly because it is set as soon as I stop heating. As I am pouring it into the casket half of it is already set. Even when I am pouring the powder slowly it is...

25 January 2023 9,146 1 View