I am trying to form chitosan nanoparticle by ionic geletion method. After mixing chitosan and tpp, do we have to filter the mixture before centrifuge?

16 August 2023 1 7K Report

After mixing the chitosan and tpp, the solution has become blurry and after filtering the mixture before centrifuge, I did not get any pellet after centrifuge.

Henry Kolge

The first step should be adding tpp dropwise into chitosan solution under stirring. The formation of pellet requires high speed, therefore you can try first to check via microcentrifuge tubes at 14000rpm

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