I could not find this information in the literature that is available to me. Any help would be greatly appreciated.
It would, but my lab has minimal funding and no experience in karyotyping (or related techniques like FISH). The A7r5 cell-line was derived decades ago, so I was hoping that someone has already carried out such a method on them. The only answer I would like is 'male' or 'female' (even better with a citation for the experimental evidence).
Forget karyotyping, too much work. PCR for the SRY gene will do the trick, only male cells have this. Alternatively if you have RNA available, a RT-PCR for the XIST female specific transcript will also work.
PS. my wife went to UCC and we got married there. Good luck!
Would not it be easier and cheaper to make your aortic culture from blood vessel from the rat you know:)?
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