I am trying to express this plasmid pGBW-m4136458 for bacterial expression of SARS-CoV-2 spike protein . How do I express it?

The size of the protein is 154KDa his-tagged. I have tried inducing with 0.5mM IPTG for 4hrs at 37 degrees. I have tried different strains BL21-De3 Gene X, shuffle T7, and artic but still no...

28 June 2023 2,428 1 View